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如何征服西方墨點法
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1.
如何征服西方墨點法
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Print date : 2025/02/13
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western blot (西方點漬法)
實驗介紹
Duration: 31:28,
Browse: 4300,
Last Updated: 2018-12-18
Play Video: http://lab.formosasoft.com/media/138
Western blotting(西方墨點法)的免疫檢驗方法,是可以強力偵測一細胞或體液中特定的微量蛋白質,這項技術是結合膠體電泳(gel electrophoresis)的優越解析力、抗體的專一性、enzyme的敏感性。如此一來,使得在一複合物中,海底撈針地找尋一蛋白質,成為可能。
Source :
https://www.youtube.com/watch?v=vBHGApaAbYg
Keypoint
Western blotting 又稱 immunoblotting,主要分成三個步驟:
1.
Sample preparation
將蛋白質混合物置於 SDS-polyacrylamide 膠體上,電泳分離,接著是 blotting: 解析的蛋白質在電場中,從膠體轉移到 Nitrocellulose transfer membrane。
2.
Electrotransfer
將 transfer membrane 浸於含有標的蛋白質的抗體(primary antibody)溶液中,形成抗原-抗體複合體。
3.
Dry transfer
藉由對 primary antibody 具專一性的 enzyme-linked 抗體(secondary antibody),來漂洗此membrane,再加入受質,產生有色物質,而偵測得知感興趣蛋白質帶。
(僅模擬資料,資料來源:
http://p5a2u2l6.pixnet.net/blog/post/30322139
)
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western blot (西方點漬法)
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western blot (西方點漬法)
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系統管理者
Branch
台灣數位學習科技
Created
2018-12-18 09:29:41
Last Updated
2018-12-18 14:59:07
Duration
31:28
Reference
1
Source
https://www.youtube.com/watch?v=vBHGApaAbYg
Media Center
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western blot (西方點漬法)
1.
實驗步驟
1.1
實驗介紹
1.2
Western Blot - Sample preparation
1.3
Western Blot - Electrophoresis
1.4
Western Blot - Semi-Dry transfer
2.
補充說明
2.1
Western Blotting Protocol
2.2
Western Blotting
3.
Western Blotting Animation - Part I